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The Academy of Integrative Medicine of Fudan University

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments
Shufen Liu 1, Yueli Sun 1, Ji Li 1, Jingcheng Dong 2, Qin Bian 1,2
1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2The Academy of Integrative Medicine of Fudan University

Here, we present the preparation of an er-xian decoction (EXD) in four steps—soaking, decoction, filtration, and concentration—and demonstrate the administration of a prepared EXD-containing serum to rats. These methods are applicable to the in vivo and in vitro study of herbal decoctions such as traditional Chinese medicines.

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Biology

A Mouse Model of Lumbar Spine Instability
Shufen Liu 1, Yueli Sun 1, Jingcheng Dong 2, Qin Bian 2
1Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Department of Integrative Medicine, Huashan Hospital, Fudan University

We developed a lumbar intervertebral disc degeneration mouse model by resection of L3–L5 spinous processes along with supra- and inter-spinous ligaments and detachment of paraspinous muscles.

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Medicine

Preparation of Naringenin Solution for In Vivo Application
Shufen Liu 1, Jingcheng Dong 2, Qin Bian 2
1Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Department of Integrative Medicine, Huashan Hospital, Fudan University

Here, the protocol presents the preparation of naringenin solution for in vivo intraperitoneal administration. Naringenin is fully dissolved in a mixture of dimethylsulfoxide, Tween 80, and saline. The antidiabetic osteoporotic effects of naringenin were assessed by blood glucose testing, tartrate-resistant acid phosphatase staining, and enzyme-linked immunosorbent assay.

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