National Clinical Research Center of Respiratory Disease
3 ARTICLES PUBLISHED IN JoVE
Medicine
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
Wenting Luo *1,2,3,4, Guichang Pan *5, Huimin Huang 1,2,3,4, Peiyan Zheng 1,2,3,4, Nili Wei 1,2,3,4, Yifei Zhang 5, Guangqiao Zeng 1,2,3,4, Baoqing Sun 1,2,3,4
1State Key Laboratory of Respiratory Disease, 2National Clinical Research Center of Respiratory Disease, 3Guangzhou Institute of Respiratory Diseases, 4Department of Allergy and Clinical Immunology, First Affiliated Hospital of Guangzhou Medical University, 5Department of Respiratory Medicine, Sixth Affiliated Hospital of Guangzhou Medical University
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
Medicine
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
Zhifeng Huang *1, Wenting Luo *1, Xiangwei Zou 1, Xiaoqing Liu 1, Chuanxu Cai 1, Zehong Wu 1, Haisheng Hu 1, Baoqing Sun 1
1Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University
This paper presents a protocol for detecting serum-specific immunoglobulin E with a microfluidic cartridge-based chemiluminescence system and the evaluation of its usefulness in allergy diagnoses.
Medicine
Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
Huiqing Zhu *1, Zhifeng Huang *1, Huimin Huang 1, Caiyou Wang 2, Liting Wu 1, Runpei Lin 1, Baoqing Sun 1
1National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, 2Laboratory Department, Fifth affiliated (Zhuhai) Hospital of Zunyi Medical University
A protocol to measure peripheral blood leukocytes using a POCT card-based leukocyte analyzer is presented here. Same blood samples were tested by two automated hematology analyzers to evaluate the consistency and accuracy of the results. The results showed that the evaluated analyzer had a good correlation with the reference system.
