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National Clinical Research Center of Respiratory Disease

3 ARTICLES PUBLISHED IN JoVE

Medicine

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Wenting Luo^*1,2,3,4, Guichang Pan^*5, Huimin Huang^1,2,3,4, Peiyan Zheng^1,2,3,4, Nili Wei^1,2,3,4, Yifei Zhang⁵, Guangqiao Zeng^1,2,3,4, Baoqing Sun^1,2,3,4

¹State Key Laboratory of Respiratory Disease, ²National Clinical Research Center of Respiratory Disease, ³Guangzhou Institute of Respiratory Diseases, ⁴Department of Allergy and Clinical Immunology, First Affiliated Hospital of Guangzhou Medical University, ⁵Department of Respiratory Medicine, Sixth Affiliated Hospital of Guangzhou Medical University

This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.

Medicine

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Zhifeng Huang^*1, Wenting Luo^*1, Xiangwei Zou¹, Xiaoqing Liu¹, Chuanxu Cai¹, Zehong Wu¹, Haisheng Hu¹, Baoqing Sun¹

¹Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University

This paper presents a protocol for detecting serum-specific immunoglobulin E with a microfluidic cartridge-based chemiluminescence system and the evaluation of its usefulness in allergy diagnoses.

Medicine

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

Huiqing Zhu^*1, Zhifeng Huang^*1, Huimin Huang¹, Caiyou Wang², Liting Wu¹, Runpei Lin¹, Baoqing Sun¹

¹National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, ²Laboratory Department, Fifth affiliated (Zhuhai) Hospital of Zunyi Medical University

A protocol to measure peripheral blood leukocytes using a POCT card-based leukocyte analyzer is presented here. Same blood samples were tested by two automated hematology analyzers to evaluate the consistency and accuracy of the results. The results showed that the evaluated analyzer had a good correlation with the reference system.