Charles River Laboratories Inc.
2 ARTICLES PUBLISHED IN JoVE
Cancer Research
Sectioning Mammary Gland Whole Mounts for Lesion Identification
Deirdre K Tucker 1,2, Julie F Foley 3, Schantel A Bouknight 4, Suzanne E Fenton 2
1Curriculum in Toxicology, University of North Carolina at Chapel Hill, 2National Toxicology Program Laboratory (NTPL), DNTP, National Institute of Environmental Health Sciences, 3Cellular and Molecular Pathology Branch, DNTP, National Institute of Environmental Health Sciences, 4Charles River Laboratories Inc.
We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.
Medicine
A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling
Leonie Durnin 1,2, Robert D. Corrigan 1, Kenton M. Sanders 1, Violeta N. Mutafova-Yambolieva 1
1Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, 2Charles River Laboratories Inc.
The detrusor-free bladder model enables direct access to the suburothelium to study local mechanisms for regulation of biologically active mediator availability in suburothelium/lamina propria during storage and voiding of urine. The preparation closely resembles filling of an intact bladder and allows pressure-volume studies to be performed without systemic influences.
