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Charles River Laboratories Inc.

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Sectioning Mammary Gland Whole Mounts for Lesion Identification

Deirdre K Tucker^1,2, Julie F Foley³, Schantel A Bouknight⁴, Suzanne E Fenton²

¹Curriculum in Toxicology, University of North Carolina at Chapel Hill, ²National Toxicology Program Laboratory (NTPL), DNTP, National Institute of Environmental Health Sciences, ³Cellular and Molecular Pathology Branch, DNTP, National Institute of Environmental Health Sciences, ⁴Charles River Laboratories Inc.

We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.

Medicine

A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling

Leonie Durnin^1,2, Robert D. Corrigan¹, Kenton M. Sanders¹, Violeta N. Mutafova-Yambolieva¹

¹Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, ²Charles River Laboratories Inc.

The detrusor-free bladder model enables direct access to the suburothelium to study local mechanisms for regulation of biologically active mediator availability in suburothelium/lamina propria during storage and voiding of urine. The preparation closely resembles filling of an intact bladder and allows pressure-volume studies to be performed without systemic influences.