Kaohsiung Medical University

7 ARTICLES PUBLISHED IN JoVE

Biology

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Wan-Shan Yang¹, Mel Campbell², Hsing-Jien Kung^2,3,4,5, Pei-Ching Chang^1,6

¹Institute of Microbiology and Immunology, National Yang-Ming University, ²UC Davis Cancer Center, University of California, Davis, ³Department of Biochemistry and Molecular Medicine, University of California, Davis, ⁴Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, ⁵Division of Molecular and Genomic Medicine, National Health Research Institutes, ⁶Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

Unlike ubiquitin ligases, few E3 SUMO ligases have been identified. This modified in vitro SUMOylation protocol is able to identify novel SUMO E3 ligases by an in vitro reconstitution assay.

Neuroscience

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Yi-Chen Lee^1,2, Shui-Chin Lu³, Yu-Lin Hsieh^1,2

¹Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Medical Research, Kaohsiung Medical University Hospital, ³Department of Medical Research, Ultrastructural Laboratory, Kaohsiung Medical University Hospital

This study establishes an experimental model of pure small fiber neuropathy with resiniferatoxin (RTX). A unique dose of RTX (50 µg/kg) is optimal for developing a small fiber neuropathy model that mimics patient characteristics and could help investigate the nociceptive molecular significance underlying neuropathic pain.

Medicine

Intra-Operative Neural Monitoring of Thyroid Surgery in a Porcine Model

Che-Wei Wu^1,2,3, Tzu-Yen Huang², Hui-Chun Chen⁴, Hsiu-Ya Chen⁵, Tsung-Yi Tsai², Pi-Ying Chang⁵, Yi-Chu Lin², Hsin-Yi Tseng², Pao-Chu Hun⁶, Xiaoli Liu⁷, Hui Sun⁷, Gregory W. Randolph⁸, Gianlorenzo Dionigi⁹, Feng-Yu Chiang^2,3, I-Cheng Lu^5,10,11

This study aims to develop a standard protocol of intra-operative neural monitoring of thyroid surgery in a porcine model. Here, we present a protocol to demonstrate general anesthesia, to compare different types of electrodes, and to investigate the electrophysiological characteristics of the normal and injured recurrent laryngeal nerves.

Biology

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Yung-Chia Chen^1,2, Bu-Miin Huang³

¹Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Anatomy, School of Medicine, Kaohsiung Medical University, ³Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University

The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis.

Medicine

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats

Hsin-Ting Lin^1,2, Yi-Lin Shiou¹, Wun-Jyun Jhuang¹, Hsiang-Chun Lee^1,3,4,5,6

¹Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, ²Hematology and Oncology, China Medical University Hospital, ³Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, ⁴Center for Lipid Biosciences, Kaohsiung Medical University Hospital, ⁵Lipid Science and Aging Research Center, Kaohsiung Medical University, ⁶Institute/Center of Medical Science and Technology, National Sun Yat-sen University

Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.

Immunology and Infection

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Jonathan Y. Wang¹, Chien-Ju Lin², Ching-Hsuan Liu^3,4, Liang-Tzung Lin^3,5

¹Department of Molecular Biosciences, University of Texas at Austin, ²School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, ³Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, ⁴Department of Microbiology and Immunology, Dalhousie University, ⁵Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University

The goal of the protocol is to illustrate the different assays relating to viral entry that can be used to identify candidate viral entry inhibitors.

Medicine

Investigation of the Electrophysiological and Thermographic Safety Parameters of Surgical Energy Devices During Thyroid and Parathyroid Surgery in a Porcine Model

Hsin-Yi Tseng¹, Tzu-Yen Huang¹, Jia Joanna Wang^1,2, Yi-Chu Lin¹, I-Cheng Lu³, Feng-Yu Chiang⁴, Gianlorenzo Dionigi^5,6, Gregory W. Randolph⁷, Che-Wei Wu^1,8

¹Department of Otorhinolaryngology-Head and Neck Surgery, International Thyroid Surgery Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, ²Department of Otolaryngology-Head and Neck Surgery, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, ³Department of Anesthesiology, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, ⁴Department of Otolaryngology-Head and Neck Surgery, E-Da Hospital, I-Shou University, ⁵Division of General Surgery, Endocrine Surgery Section, Istituto Auxologico Italiano (IRCCS), ⁶Department of Pathophysiology and Transplantation, Faculty of Medicine and Surgery, University of Milan, ⁷Division of Thyroid and Parathyroid Endocrine Surgery, Department of Otolaryngology-Head and Neck Surgery, Massachusetts Eye and Ear Infirmary, Harvard Medical School, ⁸Center for Liquid Biopsy and Cohort Research, and Faculty of Medicine, College of Medicine, Kaohsiung Medical University

The safe application of newly developed surgical energy devices in thyroid/parathyroid surgery attracts the attention of surgeons. Animal experimental models can avoid unnecessary trials and errors in human surgery. This report aims to demonstrate electrophysiological and thermographic methods to evaluate the safety parameters of SEDs in thyroid/parathyroid surgery.