University of Amsterdam, Amsterdam, The Netherlands

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Remco J. Molenaar¹, Mohammed Khurshed¹, Vashendriya V.V. Hira¹, Cornelis J.F. Van Noorden¹

¹Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.