University of Amsterdam, Amsterdam, The Netherlands

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
Remco J. Molenaar 1, Mohammed Khurshed 1, Vashendriya V.V. Hira 1, Cornelis J.F. Van Noorden 1
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.

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