Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and TissuesRemco J. Molenaar 1, Mohammed Khurshed 1, Vashendriya V.V. Hira 1, Cornelis J.F. Van Noorden 1
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.
