Yantai Zestern Biotechnique Co. LTD
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
Xiaoying Qi *1, Yunyun Zhang *2, Yuan Zhang 1, Tianhui Ni 3, Wenfeng Zhang 2, Chunhua Yang 1, Jia Mi 1,4, Jiandi Zhang 2,3, Geng Tian 1
1Medicine and Pharmacy Research Center, Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center, Binzhou Medical University, 4Department of Chemistry - BMC, Uppsala University
Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.
Medicine
A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo
Yihai Liu 1, Wenfeng Zhang 1, Tingting Hu 1, Jie Ni 1, Biao Xu 1, Wei Huang 1
1Department of Cardiology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University
Described is a protocol to establish a Doxorubicin-induced dilated cardiomyopathy (DCM) model in mice via long-term intraperitoneal injection of Doxorubicin.
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