Accedi

Yantai Zestern Biotechnique Co. LTD

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

Xiaoying Qi^*1, Yunyun Zhang^*2, Yuan Zhang¹, Tianhui Ni³, Wenfeng Zhang², Chunhua Yang¹, Jia Mi^1,4, Jiandi Zhang^2,3, Geng Tian¹

¹Medicine and Pharmacy Research Center, Binzhou Medical University, ²Yantai Zestern Biotechnique Co. LTD, ³Precision Medicine research center, Binzhou Medical University, ⁴Department of Chemistry - BMC, Uppsala University

Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.

Medicine

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo

Yihai Liu¹, Wenfeng Zhang¹, Tingting Hu¹, Jie Ni¹, Biao Xu¹, Wei Huang¹

¹Department of Cardiology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University

Described is a protocol to establish a Doxorubicin-induced dilated cardiomyopathy (DCM) model in mice via long-term intraperitoneal injection of Doxorubicin.