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First Affiliated Hospital, Xi'an Jiaotong University

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Jianwei Chen *1,2,3, Ning Shao *1,2,3, Jiaying Hu *4, Rong Li 4, Yuanshou Zhu 1,2,3, Dabing Zhang 4,5, Shujuan Guo 1,2,3, Junhou Hui 6, Peng Liu 6, Litao Yang 4, Sheng-Ce Tao 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University

This protocol describes the fabrication of a small, ready-to-use cassette that can be applied for visual detection of multiple nucleic acids in a single, test that is easy to operate. In this approach, a capillary array was used for multiplex and highly efficient detection of GMO targets.

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Medicine

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction
Lifei Yang *1,2, Jianwen Lu *1,2,3, Yue Wang 1,2,3, Mei Zhang 1,2,3, Yuan Shi 4, Shasha Wei 1,2, Peng Liu 1,2,3, Zheng Wu 3, Yi Lv 1,2,3, Rongqian Wu 1,2
1Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, First Affiliated Hospital, Xi'an Jiaotong University, 2Institute of Advanced Surgical Technology and Engineering, First Affiliated Hospital, Xi'an Jiaotong University, 3Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi'an Jiaotong University, 4Organ Transplantation Center, Tianjin First Center Hospital

The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.

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Bioengineering

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
Yingwei Wang *1,2, Cheng Lu *1,2, Chengzhi He 3, Baoxin Chen 1,2, Youling Zheng 1,2, Junming Zheng 1,2, Jianhua Zhang 4, Zheng Wu 1,2
1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, 2Department of Developmental and Regenerative Biology, Jinan University, 3Nansha College Preparatory Academy, 4Department of Cardiology, First Affiliated Hospital of Jinan University

This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property.

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Medicine

A Training and Testing System for Performing Vascular Reconstruction In Vitro
Yue Wang 1,2, Lijun Mu 3, Wei Zhang 1,2, Huan Chen 1,2, Qingshan Li 1,2, Aihua Shi 1, Bo Tang 2, Xiaogang Zhang 1,2, Dinghui Dong 1,2, Yi Lv 1,2
1National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, First Affiliated Hospital of Xi'an Jiaotong University, 2Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, 3Department of General Surgery, First Affiliated Hospital of Xi'an Jiaotong University

Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.

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Neuroscience

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats
Peng Liu *1, Xiu-Chun Song *2, Xiao-Shuai Yang 1, Qi-Long Cao 1, Yong-Yong Tang 1, Xiao-Dun Liu 3, Min Yang 1, Wen-Qiang An 1, Bai-Xiang Dong 1, Xiu-Yun Song 1
1Beijing Yinfeng Dingcheng Biological Engineering Technology Ltd., 2Hulunbuir People's Hospital, 3Shandong Qilu Stem Cell Engineering Co. Ltd.

The purpose of this study is to establish and validate an animal model for research in the recovery and sequela stages of brain ischemia by testing brain infarction and sensorimotor function after middle cerebral artery occlusion/reperfusion (MCAO/R) after 1-90 days in rats.

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