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First Affiliated Hospital, Xi'an Jiaotong University

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Jianwei Chen^*1,2,3, Ning Shao^*1,2,3, Jiaying Hu^*4, Rong Li⁴, Yuanshou Zhu^1,2,3, Dabing Zhang^4,5, Shujuan Guo^1,2,3, Junhou Hui⁶, Peng Liu⁶, Litao Yang⁴, Sheng-Ce Tao^1,2,3

¹Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, ²State Key Laboratory of Oncogenes and Related Genes, ³School of Biomedical Engineering, Shanghai Jiao Tong University, ⁴Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, ⁵Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, ⁶Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University

This protocol describes the fabrication of a small, ready-to-use cassette that can be applied for visual detection of multiple nucleic acids in a single, test that is easy to operate. In this approach, a capillary array was used for multiplex and highly efficient detection of GMO targets.

Medicine

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction

Lifei Yang^*1,2, Jianwen Lu^*1,2,3, Yue Wang^1,2,3, Mei Zhang^1,2,3, Yuan Shi⁴, Shasha Wei^1,2, Peng Liu^1,2,3, Zheng Wu³, Yi Lv^1,2,3, Rongqian Wu^1,2

¹Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, First Affiliated Hospital, Xi'an Jiaotong University, ²Institute of Advanced Surgical Technology and Engineering, First Affiliated Hospital, Xi'an Jiaotong University, ³Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi'an Jiaotong University, ⁴Organ Transplantation Center, Tianjin First Center Hospital

The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.

Bioengineering

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Yingwei Wang^*1,2, Cheng Lu^*1,2, Chengzhi He³, Baoxin Chen^1,2, Youling Zheng^1,2, Junming Zheng^1,2, Jianhua Zhang⁴, Zheng Wu^1,2

¹Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, ²Department of Developmental and Regenerative Biology, Jinan University, ³Nansha College Preparatory Academy, ⁴Department of Cardiology, First Affiliated Hospital of Jinan University

This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property.

Medicine

A Training and Testing System for Performing Vascular Reconstruction In Vitro

Yue Wang^1,2, Lijun Mu³, Wei Zhang^1,2, Huan Chen^1,2, Qingshan Li^1,2, Aihua Shi¹, Bo Tang², Xiaogang Zhang^1,2, Dinghui Dong^1,2, Yi Lv^1,2

¹National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, First Affiliated Hospital of Xi'an Jiaotong University, ²Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, ³Department of General Surgery, First Affiliated Hospital of Xi'an Jiaotong University

Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.

Neuroscience

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

Peng Liu^*1, Xiu-Chun Song^*2, Xiao-Shuai Yang¹, Qi-Long Cao¹, Yong-Yong Tang¹, Xiao-Dun Liu³, Min Yang¹, Wen-Qiang An¹, Bai-Xiang Dong¹, Xiu-Yun Song¹

¹Beijing Yinfeng Dingcheng Biological Engineering Technology Ltd., ²Hulunbuir People's Hospital, ³Shandong Qilu Stem Cell Engineering Co. Ltd.

The purpose of this study is to establish and validate an animal model for research in the recovery and sequela stages of brain ischemia by testing brain infarction and sensorimotor function after middle cerebral artery occlusion/reperfusion (MCAO/R) after 1-90 days in rats.