Universidad Pablo de Olavide
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice
Rocio Leal-Campanario 1,2, Susana Martinez-Conde 2,3, Stephen L. Macknik 2,3
1División de Neurociencias, Universidad Pablo de Olavide, 2Barrow Neurological Institute, 3Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center
Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice.
Biology
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
Juan Diego Hernández-Camacho 1,2, Cristina Vicente-García 1, Ana Sánchez-Cuesta 1,2, Daniel J. M. Fernandez-Ayala 1,2, Jaime J. Carvajal 1, Plácido Navas 1,2
1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA, Universidad Pablo de Olavide, 2CIBERER, Instituto de Salud Carlos III
Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.
