Accedi

Universidad Pablo de Olavide

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice

Rocio Leal-Campanario^1,2, Susana Martinez-Conde^2,3, Stephen L. Macknik^2,3

¹División de Neurociencias, Universidad Pablo de Olavide, ²Barrow Neurological Institute, ³Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center

Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice.

Biology

Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

Juan Diego Hernández-Camacho^1,2, Cristina Vicente-García¹, Ana Sánchez-Cuesta^1,2, Daniel J. M. Fernandez-Ayala^1,2, Jaime J. Carvajal¹, Plácido Navas^1,2

¹Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA, Universidad Pablo de Olavide, ²CIBERER, Instituto de Salud Carlos III

Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.