University Clinic of Heidelberg

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Xenia Ficht^*1, Flavian Thelen^*1, Bettina Stolp^*1,2, Jens V. Stein¹

¹Theodor-Kocher Institute, University of Bern, ²Center for Infectious Diseases, Integrative Virology, University Clinic of Heidelberg

We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.