Preparation of Murine Submandibular Salivary Gland for Upright Intravital MicroscopyXenia Ficht *1, Flavian Thelen *1, Bettina Stolp *1,2, Jens V. Stein 1
1Theodor-Kocher Institute, University of Bern, 2Center for Infectious Diseases, Integrative Virology, University Clinic of Heidelberg
We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.
