Removal of the Fur and Fixation of the Mouse on the Stage
2:46
Surgical Exposure of the Salivary Gland Using a Stereomicroscope
4:09
Fixing of the Gland, Heating Ring, and the Temperature Probe
6:10
Results: Use of Immunofluorescence Imaging to Study the Submandibular Gland
7:19
Conclusion
副本
The overall goal of this surgical procedure is to prepare the murine submandibular salivary gland for upright intravital microscopy. This method can help answer questions in any field of research that uses the submandibular salivary gland as a mod
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We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.