The University of Texas at Arlington
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
Victoria Messerschmidt *1, Zachary Bailey *1, Kyung In Baek 2, Yichen Ding 2, Jeffrey J. Hsu 2, Richard Bryant 1, Rongsong Li 3, Tzung K. Hsiai 2, Juhyun Lee 1
1Department of Bioengineering, The University of Texas at Arlington, 2Department of Medicine (Cardiology) and Bioengineering, UCLA, 3College of Health Science and Environmental Engineering, Shenzhen Technology University
Here, we present a protocol to visualize developing hearts in zebrafish in 4-Dimensions (4-D). 4-D imaging, via light-sheet fluorescence microscopy (LSFM), takes 3-Dimensional (3-D) images over time, to reconstruct developing hearts. We show qualitatively and quantitatively that shear stress activates endocardial Notch signaling during chamber development, which promotes cardiac trabeculation.
Bioengineering
Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
Yichen Ding 1, Zachary Bailey 2, Victoria Messerschmidt 2, Jun Nie 3, Richard Bryant 2, Sandra Rugonyi 4, Peng Fei 3, Juhyun Lee 1,2, Tzung K. Hsiai 1
1Department of Bioengineering, University of California Los Angeles, 2Department of Bioengineering, University of Texas at Arlington, 3School of Optical and Electronic Information, Huazhong University of Science and Technology, 4Department of Biomedical Engineering, OSHU
This study uses a dual-sided illumination light-sheet fluorescence microscopy (LSFM) technique combined with optical clearing to study the murine heart.
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