The University of Texas at Arlington

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Victoria Messerschmidt^*1, Zachary Bailey^*1, Kyung In Baek², Yichen Ding², Jeffrey J. Hsu², Richard Bryant¹, Rongsong Li³, Tzung K. Hsiai², Juhyun Lee¹

¹Department of Bioengineering, The University of Texas at Arlington, ²Department of Medicine (Cardiology) and Bioengineering, UCLA, ³College of Health Science and Environmental Engineering, Shenzhen Technology University

Here, we present a protocol to visualize developing hearts in zebrafish in 4-Dimensions (4-D). 4-D imaging, via light-sheet fluorescence microscopy (LSFM), takes 3-Dimensional (3-D) images over time, to reconstruct developing hearts. We show qualitatively and quantitatively that shear stress activates endocardial Notch signaling during chamber development, which promotes cardiac trabeculation.

Bioengineering

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Yichen Ding¹, Zachary Bailey², Victoria Messerschmidt², Jun Nie³, Richard Bryant², Sandra Rugonyi⁴, Peng Fei³, Juhyun Lee^1,2, Tzung K. Hsiai¹

¹Department of Bioengineering, University of California Los Angeles, ²Department of Bioengineering, University of Texas at Arlington, ³School of Optical and Electronic Information, Huazhong University of Science and Technology, ⁴Department of Biomedical Engineering, OSHU

This study uses a dual-sided illumination light-sheet fluorescence microscopy (LSFM) technique combined with optical clearing to study the murine heart.