Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands
3 ARTICLES PUBLISHED IN JoVE
Neuroscience
In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
Rosa Crespo 1, Wouter Koudstaal 1, Adrian Apetri 1
1Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson
The tau aggregation assay described in this manuscript mimics the anticipated features of in vivo tau misfolding and aggregation.
Neuroscience
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
Donata De Marco 1, Renske Taggenbrock 2,3, Rosa Crespo 3, Wouter Koudstaal 3, Elizabeth Ramsburg 4, Adrian Apetri 3
1Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania
Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.
Immunology and Infection
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Stuart T. Perry 1, Elissa Keogh 1, Mike Morton 1, Wouter Koudstaal 2, Gabriel Pascual 1
1World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson
The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.
