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Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Rosa Crespo¹, Wouter Koudstaal¹, Adrian Apetri¹

¹Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

The tau aggregation assay described in this manuscript mimics the anticipated features of in vivo tau misfolding and aggregation.

Neuroscience

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

Donata De Marco¹, Renske Taggenbrock^2,3, Rosa Crespo³, Wouter Koudstaal³, Elizabeth Ramsburg⁴, Adrian Apetri³

¹Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, ²Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, ³Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, ⁴Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.

Immunology and Infection

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Stuart T. Perry¹, Elissa Keogh¹, Mike Morton¹, Wouter Koudstaal², Gabriel Pascual¹

¹World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, ²Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.