Qingdao Agricultural University, Qingdao, China
2 ARTICLES PUBLISHED IN JoVE
Medicine
Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging
Ed Lim 1, Kshitij Modi 1, Anna Christensen 1, Jeff Meganck 1, Stephen Oldfield 1, Ning Zhang 1
1Imaging Biology Research and Development, Caliper Life Sciences
An experimental mouse model of bone metastasis was established following intracardiac delivery of luciferase expressing mammary tumor cells. Tumor development and resulted osteolytic lesion were monitored longitudinally with bioluminescence and micro CT imaging.
Bioengineering
Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System
Hegang Li *1, Huaiyuan Qin *1, Ning Zhang *1, Jinshan Zhao 1, Jingjing Xin 1, Flor M. Perez-Campo 2, Huawei Liu 1
1Qingdao Agricultural University, Qingdao, China, 2University of Cantabria, Santander y Torrelavega, Spain
Here, we present a protocol describing a streamlined method for the efficient generation of plasmids expressing both the CRISPR enzyme and associated single guide RNA (sgRNAs). Co-transfection of mammalian cells with this sgRNA/CRISPR vector and a dual luciferase reporter vector that examines double-strand break repair allows evaluation of knockout efficiency.
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