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Qingdao Agricultural University, Qingdao, China

2 ARTICLES PUBLISHED IN JoVE

Medicine

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Ed Lim¹, Kshitij Modi¹, Anna Christensen¹, Jeff Meganck¹, Stephen Oldfield¹, Ning Zhang¹

¹Imaging Biology Research and Development, Caliper Life Sciences

An experimental mouse model of bone metastasis was established following intracardiac delivery of luciferase expressing mammary tumor cells. Tumor development and resulted osteolytic lesion were monitored longitudinally with bioluminescence and micro CT imaging.

Bioengineering

Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System

Hegang Li^*1, Huaiyuan Qin^*1, Ning Zhang^*1, Jinshan Zhao¹, Jingjing Xin¹, Flor M. Perez-Campo², Huawei Liu¹

¹Qingdao Agricultural University, Qingdao, China, ²University of Cantabria, Santander y Torrelavega, Spain

Here, we present a protocol describing a streamlined method for the efficient generation of plasmids expressing both the CRISPR enzyme and associated single guide RNA (sgRNAs). Co-transfection of mammalian cells with this sgRNA/CRISPR vector and a dual luciferase reporter vector that examines double-strand break repair allows evaluation of knockout efficiency.