University of Ulsan College of Medicine/Asan Medical Center
2 ARTICLES PUBLISHED IN JoVE
Biology
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells
Jeong Hoon Lee 1, Jeong Mi Ha 1, Quynh Mai Ho 1, Chae Hun Leem 1,2,3
1Department of Physiology, University of Ulsan College of Medicine/Asan Medical Center, 2Asan Medical Center, 3Asan Medical Institute of Convergence Science and Technology
Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca2+]. Thus, a novel online correction method of NADH signal interference to measure [Ca2+] was developed.
Biochemistry
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
Dohwan Lee *1, Kyung Wook Wee *1, Hyerin Kim *1, Sung Il Han 1, Seungmin Kwak 1,2, Dae Sung Yoon 3, Jeong Hoon Lee 1
1Department of Electrical Engineering, Kwangwoon University, 2Korea Institute of Science and Technology (KIST), 3School of Biomedical Engineering, Korea University
Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes.
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