Accedi

University of Ulsan College of Medicine/Asan Medical Center

2 ARTICLES PUBLISHED IN JoVE

Biology

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca²⁺ Measurement with Fura-2-Analog in Live Cells

Jeong Hoon Lee¹, Jeong Mi Ha¹, Quynh Mai Ho¹, Chae Hun Leem^1,2,3

¹Department of Physiology, University of Ulsan College of Medicine/Asan Medical Center, ²Asan Medical Center, ³Asan Medical Institute of Convergence Science and Technology

Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca²⁺]. Thus, a novel online correction method of NADH signal interference to measure [Ca²⁺] was developed.

Biochemistry

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Dohwan Lee^*1, Kyung Wook Wee^*1, Hyerin Kim^*1, Sung Il Han¹, Seungmin Kwak^1,2, Dae Sung Yoon³, Jeong Hoon Lee¹

¹Department of Electrical Engineering, Kwangwoon University, ²Korea Institute of Science and Technology (KIST), ³School of Biomedical Engineering, Korea University

Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes.