National Institute of Environmental Health and Sciences
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
Jennifer H. Madenspacher 1, Michael B. Fessler 1
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health
Several methods have been described in the literature for modeling bacterial pneumonia in mice. Herein, we describe a non-invasive, inexpensive, rapid method for inducing pneumonia via aspiration (i.e., inhalation) of a bacterial inoculum pipetted into the oropharynx. Downstream methods for assessment of the pulmonary innate immune response are also detailed.
Immunology and Infection
In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
Myles X. Hodge 1, Sky W. Reece 1, Jennifer H. Madenspacher 2, Kymberly M. Gowdy 1
1Department of Pharmacology and Toxicology, East Carolina University, 2Research Triangle Park, National Institute of Environmental Health and Sciences
This manuscript describes a protocol for determining whether exposure to ozone, a criteria air pollutant, impairs alveolar macrophage efferocytosis in vivo. This protocol utilizes commonly used reagents and techniques and can be adapted to multiple models of pulmonary injury to determine effects on alveolar macrophage efferocytosis.
