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National Institute of Environmental Health and Sciences

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

Jennifer H. Madenspacher¹, Michael B. Fessler¹

¹Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health

Several methods have been described in the literature for modeling bacterial pneumonia in mice. Herein, we describe a non-invasive, inexpensive, rapid method for inducing pneumonia via aspiration (i.e., inhalation) of a bacterial inoculum pipetted into the oropharynx. Downstream methods for assessment of the pulmonary innate immune response are also detailed.

Immunology and Infection

In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure

Myles X. Hodge¹, Sky W. Reece¹, Jennifer H. Madenspacher², Kymberly M. Gowdy¹

¹Department of Pharmacology and Toxicology, East Carolina University, ²Research Triangle Park, National Institute of Environmental Health and Sciences

This manuscript describes a protocol for determining whether exposure to ozone, a criteria air pollutant, impairs alveolar macrophage efferocytosis in vivo. This protocol utilizes commonly used reagents and techniques and can be adapted to multiple models of pulmonary injury to determine effects on alveolar macrophage efferocytosis.