Grupo Boticário
3 ARTICLES PUBLISHED IN JoVE
JoVE Core
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Talita Miguel Marin 1, Nathalia de Carvalho Indolfo 4, Silvana Aparecida Rocco 1, Murilo de Carvalho 1,2, Marilia Meira Dias 1, Graziele Izalina Vasconcelos Bento 1, Leandro Oliveira Bortot 1, Desirée Cigaran Schuck 3, Márcio Lorencini 3, Eduardo Pagani 1
1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), 2Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 3Grupo Boticário, 4Institute of Biology, University of Campinas
We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
Environment
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
Irisdoris Rodrigues de Souza 1, Andrezza Di Pietro Micali Canavez 2, Desiree Cigaran Schuck 2, Viviana Stephanie Costa Gagosian 1, Isisdoris Rodrigues de Souza 1, Natália de Albuquerque Vita 2, Edvaldo da Silva Trindade 3, Marta Margarete Cestari 4, Marcio Lorencini 2, Daniela Morais Leme 4
1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Cell Biology, Federal University of Paraná (UFPR), 4Department of Genetics, Federal University of Paraná (UFPR)
Here, we present an effective, easy, and fast 3D culture protocol for the formation of spheroids of two zebrafish (Danio rerio) cell lines: ZEM2S (embryo) and ZFL (normal hepatocyte).
Environment
Cytotoxicity Assays with Zebrafish Cell Lines
Irisdoris Rodrigues de Souza 1, Tainá Wilke Sivek 1, Júlia Beatriz Vaz de Oliveira 1, Andrezza Di Pietro Micali Canavez 2, Natália de Albuquerque Vita 2, Desiree Cigaran Schuck 2, Isisdoris Rodrigues de Souza 1, Marta Margarete Cestari 3, Marcio Lorencini 2, Daniela Morais Leme 3
1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Genetics, Federal University of Paraná (UFPR)
This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.
