INSERM U932
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Study of Cell Migration in Microfabricated Channels
Pablo Vargas 1, Emmanuel Terriac 2, Ana-Maria Lennon-Duménil 1, Matthieu Piel 2
1Immunité et Cancer, Institut Curie, 2Compartimentation et Dynamique Cellulaires, Institut Curie
A quantitative method to study spontaneous migration of cells in a one-dimensional confined microenvironment is described. This method takes advantage of microfabricated channels and can be used to study migration of large number of cells under different conditions in single experiments.
Bioengineering
Traction Force Microscopy to Study B Lymphocyte Activation
Anita Kumari 1, Judith Pineau 1, Ana-Maria Lennon-Duménil 1, Martial Balland 2, Paolo Pierobon 1
1Institut Curie, PSL Research University, INSERM U932, 2Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1)
Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.
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