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INSERM U932

2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Study of Cell Migration in Microfabricated Channels
Pablo Vargas 1, Emmanuel Terriac 2, Ana-Maria Lennon-Duménil 1, Matthieu Piel 2
1Immunité et Cancer, Institut Curie, 2Compartimentation et Dynamique Cellulaires, Institut Curie

A quantitative method to study spontaneous migration of cells in a one-dimensional confined microenvironment is described. This method takes advantage of microfabricated channels and can be used to study migration of large number of cells under different conditions in single experiments.

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Bioengineering

Traction Force Microscopy to Study B Lymphocyte Activation
Anita Kumari 1, Judith Pineau 1, Ana-Maria Lennon-Duménil 1, Martial Balland 2, Paolo Pierobon 1
1Institut Curie, PSL Research University, INSERM U932, 2Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1)

Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.

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