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INSERM U932

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Study of Cell Migration in Microfabricated Channels

Pablo Vargas¹, Emmanuel Terriac², Ana-Maria Lennon-Duménil¹, Matthieu Piel²

¹Immunité et Cancer, Institut Curie, ²Compartimentation et Dynamique Cellulaires, Institut Curie

A quantitative method to study spontaneous migration of cells in a one-dimensional confined microenvironment is described. This method takes advantage of microfabricated channels and can be used to study migration of large number of cells under different conditions in single experiments.

Bioengineering

Traction Force Microscopy to Study B Lymphocyte Activation

Anita Kumari¹, Judith Pineau¹, Ana-Maria Lennon-Duménil¹, Martial Balland², Paolo Pierobon¹

¹Institut Curie, PSL Research University, INSERM U932, ²Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1)

Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.