McGovern Medical School at UT Health
2 ARTICLES PUBLISHED IN JoVE
Biology
Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
Askar M. Akimzhanov 1, Darren Boehning 1
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch
This protocol describes a method for real-time measurement of mitochondrial calcium fluxes by fluorescent imaging. The method takes advantage of a circularly permutated YFP-based dual-excitation ratiometric calcium sensor (ratiometric pericam-mt) selectively expressed in mitochondria.
Biochemistry
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
Ritika Tewari *1, Savannah J. West *1,2, Bieerkehazi Shayahati 1, Askar M. Akimzhanov 1
1Department of Biochemistry and Molecular Biology, McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School
Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological samples.
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