STEMCELL Technologies Inc
2 ARTICLES PUBLISHED IN JoVE
Biology
Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
Jose Meseguer-Ripolles 1, Yu Wang 1, Agnes Sorteberg 1, Aishwariya Sharma 2, Nan-Linda Ding 2, Baltasar Lucendo-Villarin 1, Philipp Kramer 2, Charis-Patricia Segeritz 2, David C. Hay 1
1MRC Centre for Regenerative Medicine, University of Edinburgh, 2Research & Development, STEMCELL Technologies Inc
The goal of this article is to provide a standardized approach to induce human hepatic progenitor differentiation from pluripotent stem cells. The development of this procedure with ready-to-use media formulations offer the user a facile system to generate human liver cells for biomedical research and translation.
Developmental Biology
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters
Jia Zhao *1, Shenghui Liang *1, Mitchell J. S. Braam 1, Robert K. Baker 1, Diepiriye G. Iworima 1,2, Nina Quiskamp 3, Timothy J. Kieffer 1,2,4
1Department of Cellular & Physiological Sciences, Life Science Institute, University of British Columbia, 2School of Biomedical Engineering, University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery, University of British Columbia
The differentiation of stem cells into islet cells provides an alternative solution to conventional diabetes treatment and disease modeling. We describe a detailed stem cell culture protocol that combines a commercial differentiation kit with a previously validated method to aid in producing insulin-secreting, stem cell-derived islets in a dish.
