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STEMCELL Technologies Inc

2 ARTICLES PUBLISHED IN JoVE

Biology

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Jose Meseguer-Ripolles¹, Yu Wang¹, Agnes Sorteberg¹, Aishwariya Sharma², Nan-Linda Ding², Baltasar Lucendo-Villarin¹, Philipp Kramer², Charis-Patricia Segeritz², David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh, ²Research & Development, STEMCELL Technologies Inc

The goal of this article is to provide a standardized approach to induce human hepatic progenitor differentiation from pluripotent stem cells. The development of this procedure with ready-to-use media formulations offer the user a facile system to generate human liver cells for biomedical research and translation.

Developmental Biology

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Jia Zhao^*1, Shenghui Liang^*1, Mitchell J. S. Braam¹, Robert K. Baker¹, Diepiriye G. Iworima^1,2, Nina Quiskamp³, Timothy J. Kieffer^1,2,4

¹Department of Cellular & Physiological Sciences, Life Science Institute, University of British Columbia, ²School of Biomedical Engineering, University of British Columbia, ³STEMCELL Technologies Inc, ⁴Department of Surgery, University of British Columbia

The differentiation of stem cells into islet cells provides an alternative solution to conventional diabetes treatment and disease modeling. We describe a detailed stem cell culture protocol that combines a commercial differentiation kit with a previously validated method to aid in producing insulin-secreting, stem cell-derived islets in a dish.