University Hospital Basel and University of Basel
2 ARTICLES PUBLISHED IN JoVE
Medicine
Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
Hui Wang 1,2, Anna Paczulla 1, Claudia Lengerke 1,2
1Department of Biomedicine, University Hospital Basel, 2Department of Internal Medicine II, University Hospital Tübingen
In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.
Cancer Research
Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
Henrik Landerer *1, Marlon Arnone *1, Ronja Wieboldt 2, Elsa Goersch 1, Anna M. Paczulla Stanger 3, Martina Konantz 1, Claudia Lengerke 1,2,3
1Department of Biomedicine, University Hospital Basel and University of Basel, 2Division for Hematology, University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen
We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.
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