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University Hospital Basel and University of Basel

2 ARTICLES PUBLISHED IN JoVE

Medicine

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Hui Wang^1,2, Anna Paczulla¹, Claudia Lengerke^1,2

¹Department of Biomedicine, University Hospital Basel, ²Department of Internal Medicine II, University Hospital Tübingen

In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

Cancer Research

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Henrik Landerer^*1, Marlon Arnone^*1, Ronja Wieboldt², Elsa Goersch¹, Anna M. Paczulla Stanger³, Martina Konantz¹, Claudia Lengerke^1,2,3

¹Department of Biomedicine, University Hospital Basel and University of Basel, ²Division for Hematology, University Hospital Basel and University of Basel, ³Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen

We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.