Leibniz University Hannover
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Changhua Zhu *1, Xiaoye Liu *2, Wenlei Wang 1, Xiaoguang Chen 3, Shangyu Gao 1, Meng Qian 1, Na Yang 1, Yifeng Xu 1, Mingjia Chen 1
1College of Life Sciences, Nanjing Agricultural University, 2Department of Criminal Science and Technology, Nanjing Forest Police College, 3Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition, Leibniz University Hannover
A precise and reproducible method for in vivo nucleosides/nucleotides quantification in plants is described here. This method employs an HPLC-MS/MS.
Immunology and Infection
Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay
Jamila Franca Rosengarten *1,2, Stefanie Schatz *1,2, Jörn Stitz 1
1Research Group Pharmaceutical Biotechnology, TH Köln - University of Applied Sciences, 2Institute of Technical Chemistry, Leibniz University Hannover
Here, we present a protocol to detect neutralization epitopes on antigen-displaying virus-like particles (VLPs). Immunoprecipitation of the human immunodeficiency virus (HIV)-derived VLPs is performed using envelope glycoproteins-specific monoclonal antibodies coupled to protein G-conjugated magnetic beads. Captured VLPs are subsequently subjected to SDS-PAGE and Western blot-analysis employing viral core protein Gag-specific antibodies.
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