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Leibniz University Hannover

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Changhua Zhu^*1, Xiaoye Liu^*2, Wenlei Wang¹, Xiaoguang Chen³, Shangyu Gao¹, Meng Qian¹, Na Yang¹, Yifeng Xu¹, Mingjia Chen¹

¹College of Life Sciences, Nanjing Agricultural University, ²Department of Criminal Science and Technology, Nanjing Forest Police College, ³Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition, Leibniz University Hannover

A precise and reproducible method for in vivo nucleosides/nucleotides quantification in plants is described here. This method employs an HPLC-MS/MS.

Immunology and Infection

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

Jamila Franca Rosengarten^*1,2, Stefanie Schatz^*1,2, Jörn Stitz¹

¹Research Group Pharmaceutical Biotechnology, TH Köln - University of Applied Sciences, ²Institute of Technical Chemistry, Leibniz University Hannover

Here, we present a protocol to detect neutralization epitopes on antigen-displaying virus-like particles (VLPs). Immunoprecipitation of the human immunodeficiency virus (HIV)-derived VLPs is performed using envelope glycoproteins-specific monoclonal antibodies coupled to protein G-conjugated magnetic beads. Captured VLPs are subsequently subjected to SDS-PAGE and Western blot-analysis employing viral core protein Gag-specific antibodies.