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University Medical Center of the Johannes Gutenberg-University of Mainz

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
Jeremy Lagrange 1, Sabine Kossmann 1,2, Klytaimnistra Kiouptsi 1, Philip Wenzel 1,2,3
1Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, 2Center for Cardiology, University Medical Center Mainz, 3German Center for Cardiovascular Research (DZHK)

This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.

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Neuroscience

A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage
Axel Neulen *1, Michael Kosterhon *1, Tobias Pantel *1, Stefanie Kirschner 2, Hermann Goetz 3, Marc A. Brockmann 2, Sven R. Kantelhardt 1, Serge C. Thal 4
1Department of Neurosurgery, Medical Center of the Johannes Gutenberg - University, 2Department of Neuroradiology, Medical Center of the Johannes Gutenberg - University, 3Platform for Biomaterial Research, Medical Center of the Johannes Gutenberg - University, 4Department of Anesthesiology, Medical Center of the Johannes Gutenberg - University

The goal of this article is to present a method that allows a 3-dimensional reconstruction of the cerebrovascular tree in mice after micro computed tomography and determination of volumes of entire vessel segments that can be used to quantify cerebral vasospasm in murine models of subarachnoid hemorrhage.

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Medicine

Multicolor 3D Printing of Complex Intracranial Tumors in Neurosurgery
Michael Kosterhon 1, Meik Neufurth 2, Axel Neulen 1, Lea Schäfer 1, Jens Conrad 1, Sven R. Kantelhardt 1, Werner E. G. Müller 2, Florian Ringel 1
1Department of Neurosurgery, University Medical Center of the Johannes Gutenberg University Mainz, Germany, 2Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Germany

The protocol describes the fabrication of fully colored three-dimensional prints of patient-specific, anatomical skull models to be used for surgical simulation. The crucial steps of combining different imaging modalities, image segmentation, three-dimensional model extraction, and production of the prints are explained.

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Neuroscience

Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound
Axel Neulen *1, Michael Molitor *2,3,4, Michael Kosterhon *1, Tobias Pantel 1, Susanne H. Karbach 2,3,4, Philip Wenzel 2,3,4, Thomas Gaul 5, Florian Ringel 1, Serge C. Thal 5
1Department of Neurosurgery, University Medical Center of the Johannes Gutenberg-University of Mainz, 2Center for Cardiology - Cardiology I, University Medical Center of the Johannes Gutenberg-University of Mainz, 3Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University of Mainz, 4German Center for Cardiovascular Research (DZHK) - Partner site Rhine-Main, 5Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University of Mainz

The aim of this manuscript is to present a sonography-based method that allows in vivo imaging of blood flow in cerebral arteries in mice. We demonstrate its application to determine changes in blood flow velocities associated with vasospasm in murine models of subarachnoid hemorrhage (SAH).

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