University Medical Center of the Johannes Gutenberg-University of Mainz

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls

Jeremy Lagrange¹, Sabine Kossmann^1,2, Klytaimnistra Kiouptsi¹, Philip Wenzel^1,2,3

¹Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, ²Center for Cardiology, University Medical Center Mainz, ³German Center for Cardiovascular Research (DZHK)

This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.

Neuroscience

A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage

Axel Neulen^*1, Michael Kosterhon^*1, Tobias Pantel^*1, Stefanie Kirschner², Hermann Goetz³, Marc A. Brockmann², Sven R. Kantelhardt¹, Serge C. Thal⁴

¹Department of Neurosurgery, Medical Center of the Johannes Gutenberg - University, ²Department of Neuroradiology, Medical Center of the Johannes Gutenberg - University, ³Platform for Biomaterial Research, Medical Center of the Johannes Gutenberg - University, ⁴Department of Anesthesiology, Medical Center of the Johannes Gutenberg - University

The goal of this article is to present a method that allows a 3-dimensional reconstruction of the cerebrovascular tree in mice after micro computed tomography and determination of volumes of entire vessel segments that can be used to quantify cerebral vasospasm in murine models of subarachnoid hemorrhage.

Medicine

Multicolor 3D Printing of Complex Intracranial Tumors in Neurosurgery

Michael Kosterhon¹, Meik Neufurth², Axel Neulen¹, Lea Schäfer¹, Jens Conrad¹, Sven R. Kantelhardt¹, Werner E. G. Müller², Florian Ringel¹

¹Department of Neurosurgery, University Medical Center of the Johannes Gutenberg University Mainz, Germany, ²Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Germany

The protocol describes the fabrication of fully colored three-dimensional prints of patient-specific, anatomical skull models to be used for surgical simulation. The crucial steps of combining different imaging modalities, image segmentation, three-dimensional model extraction, and production of the prints are explained.

Neuroscience

Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound

Axel Neulen^*1, Michael Molitor^*2,3,4, Michael Kosterhon^*1, Tobias Pantel¹, Susanne H. Karbach^2,3,4, Philip Wenzel^2,3,4, Thomas Gaul⁵, Florian Ringel¹, Serge C. Thal⁵

¹Department of Neurosurgery, University Medical Center of the Johannes Gutenberg-University of Mainz, ²Center for Cardiology - Cardiology I, University Medical Center of the Johannes Gutenberg-University of Mainz, ³Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University of Mainz, ⁴German Center for Cardiovascular Research (DZHK) - Partner site Rhine-Main, ⁵Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University of Mainz

The aim of this manuscript is to present a sonography-based method that allows in vivo imaging of blood flow in cerebral arteries in mice. We demonstrate its application to determine changes in blood flow velocities associated with vasospasm in murine models of subarachnoid hemorrhage (SAH).