Kenya Medical Research Institute

1 ARTICLES PUBLISHED IN JoVE

Biology

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Raphael Nyaruaba^1,2,3, Xiaohong Li¹, Caroline Mwaliko^2,3,4, Changchang Li¹, Matilu Mwau⁵, Nelson Odiwour^1,2,3, Elishiba Muturi^1,2,3, Caroline Muema^1,2,3, Junhua Li¹, Junping Yu¹, Hongping Wei¹

¹Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, ²International College, University of Chinese Academy of Sciences, ³Sino-Africa Joint Research Center, ⁴CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, ⁵Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

This work summarizes steps on developing different assays for SARS-CoV-2 detection using a two color ddPCR system. The steps are elaborate and notes have been included on how to improve the assays and experiment performance. These assays may be used for multiple SARS-CoV-2 RT-ddPCR applications.