Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and QuantificationRaphael Nyaruaba 1,2,3, Xiaohong Li 1, Caroline Mwaliko 2,3,4, Changchang Li 1, Matilu Mwau 5, Nelson Odiwour 1,2,3, Elishiba Muturi 1,2,3, Caroline Muema 1,2,3, Junhua Li 1, Junping Yu 1, Hongping Wei 1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute
This work summarizes steps on developing different assays for SARS-CoV-2 detection using a two color ddPCR system. The steps are elaborate and notes have been included on how to improve the assays and experiment performance. These assays may be used for multiple SARS-CoV-2 RT-ddPCR applications.
