Instituto de Investigación Sanitaria del Principado de Asturias (ISPA)
2 ARTICLES PUBLISHED IN JoVE
Biology
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
Andrea Acebes-Huerta 1, Patricia Martínez-Botía 1, Cristina Martín Martín 2, Ángel Bernardo 1,3, Adrián R. Rodríguez 1, María Carmen Vicente-Ayuso 4, Celina Benavente Cuesta 5, Laura Gutiérrez 1,6
1Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 2Flow Cytometry and Cell Sorting Platform (ISPA), 3Clinical Diagnosis Laboratory - Dept. of Hematology, Hospital Universitario Central de Asturias (HUCA), 4Department of Hematology, Hospital Universitario Severo Ochoa (HUSO), 5Department of Hematology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), 6Dept. of Medicine, University of Oviedo
Here, we present an immunophenotyping strategy for the characterization of megakaryocyte differentiation, and show how that strategy allows the sorting of megakaryocytes at different stages with a fluorescence-activated cell sorter. The methodology can be applied to human primary tissues, but also to megakaryocytes generated in culture in vitro.
Biology
Versatility of Protocols for Resistance Training and Assessment Using Static and Dynamic Ladders in Animal Models
Eduardo Iglesias-Gutiérrez 1,2, Manuel Fernández-Sanjurjo 2, Álvaro F. Fernández 3, Francisco José Rodríguez Díaz 4, Isabel López-Taboada 5,6, Cristina Tomás-Zapico 1,2, Benjamín Fernández-García 2,7
1Department of Functional Biology, University of Oviedo, 2Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 3Department of Biochemistry and Molecular Biology, University of Oviedo, 4Animal facilities, University of Oviedo, 5Department of Psychology, University of Oviedo, 6Instituto de Neurociencias del Principado de Asturias (INEUROPA), University of Oviedo, 7Department of Morphology and Cellular Biology, University of Oviedo
The present protocol describes resistance training and testing using static and dynamic ladders in animal models.
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