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Instituto de Investigación Sanitaria del Principado de Asturias (ISPA)

2 ARTICLES PUBLISHED IN JoVE

Biology

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Andrea Acebes-Huerta¹, Patricia Martínez-Botía¹, Cristina Martín Martín², Ángel Bernardo^1,3, Adrián R. Rodríguez¹, María Carmen Vicente-Ayuso⁴, Celina Benavente Cuesta⁵, Laura Gutiérrez^1,6

¹Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ²Flow Cytometry and Cell Sorting Platform (ISPA), ³Clinical Diagnosis Laboratory - Dept. of Hematology, Hospital Universitario Central de Asturias (HUCA), ⁴Department of Hematology, Hospital Universitario Severo Ochoa (HUSO), ⁵Department of Hematology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), ⁶Dept. of Medicine, University of Oviedo

Here, we present an immunophenotyping strategy for the characterization of megakaryocyte differentiation, and show how that strategy allows the sorting of megakaryocytes at different stages with a fluorescence-activated cell sorter. The methodology can be applied to human primary tissues, but also to megakaryocytes generated in culture in vitro.

Biology

Versatility of Protocols for Resistance Training and Assessment Using Static and Dynamic Ladders in Animal Models

Eduardo Iglesias-Gutiérrez^1,2, Manuel Fernández-Sanjurjo², Álvaro F. Fernández³, Francisco José Rodríguez Díaz⁴, Isabel López-Taboada^5,6, Cristina Tomás-Zapico^1,2, Benjamín Fernández-García^2,7

¹Department of Functional Biology, University of Oviedo, ²Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ³Department of Biochemistry and Molecular Biology, University of Oviedo, ⁴Animal facilities, University of Oviedo, ⁵Department of Psychology, University of Oviedo, ⁶Instituto de Neurociencias del Principado de Asturias (INEUROPA), University of Oviedo, ⁷Department of Morphology and Cellular Biology, University of Oviedo

The present protocol describes resistance training and testing using static and dynamic ladders in animal models.