Centre for Eye and Vision Research (CEVR)
2 ARTICLES PUBLISHED IN JoVE
Biology
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
Jaclyn M. L. Chang 1, Junghee Seo 1, Maggie Miu Yee Kwan 1, Sarah Oh 1, David J. McCanna 1, Lakshman Subbaraman 1, Lyndon Jones 1,2
1Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science, University of Waterloo, 2Centre for Eye and Vision Research (CEVR)
This article describes the procedures used to evaluate the toxicity of UV radiation and chemical toxins on a primary and immortalized cell line.
Medicine
A Protein Suspension-Trapping Sample Preparation for Tear Proteomics by Liquid Chromatography-Tandem Mass Spectrometry
Jimmy Sung-Hei Tse *1, Ying-Hon Sze *1,2, Jimmy Ka-Wai Cheung 1,2, King-Kit Li 1, Thomas Chuen Lam 1,2,3,4
1Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, 2Centre for Eye and Vision Research (CEVR), 3Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, 4Research Centre for Chinese Medicine Innovation (RCMI), The Hong Kong Polytechnic University
This protocol describes a method for collecting tear samples using Schirmer strips and an integrated quantitative workflow for discovering non-invasive tear protein biomarkers. The suspension trapping sample preparation workflow enables fast and robust tear sample preparation and mass spectrometric analysis, resulting in higher peptide recovery yields and protein identification than standard in-solution procedures.
