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Centre for Eye and Vision Research (CEVR)

2 ARTICLES PUBLISHED IN JoVE

Biology

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

Jaclyn M. L. Chang¹, Junghee Seo¹, Maggie Miu Yee Kwan¹, Sarah Oh¹, David J. McCanna¹, Lakshman Subbaraman¹, Lyndon Jones^1,2

¹Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science, University of Waterloo, ²Centre for Eye and Vision Research (CEVR)

This article describes the procedures used to evaluate the toxicity of UV radiation and chemical toxins on a primary and immortalized cell line.

Medicine

A Protein Suspension-Trapping Sample Preparation for Tear Proteomics by Liquid Chromatography-Tandem Mass Spectrometry

Jimmy Sung-Hei Tse^*1, Ying-Hon Sze^*1,2, Jimmy Ka-Wai Cheung^1,2, King-Kit Li¹, Thomas Chuen Lam^1,2,3,4

¹Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, ²Centre for Eye and Vision Research (CEVR), ³Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, ⁴Research Centre for Chinese Medicine Innovation (RCMI), The Hong Kong Polytechnic University

This protocol describes a method for collecting tear samples using Schirmer strips and an integrated quantitative workflow for discovering non-invasive tear protein biomarkers. The suspension trapping sample preparation workflow enables fast and robust tear sample preparation and mass spectrometric analysis, resulting in higher peptide recovery yields and protein identification than standard in-solution procedures.