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Einstein College of Medicine / Montefiore Medical Center

5 ARTICLES PUBLISHED IN JoVE

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Medicine

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment
Allison S. Harney 1,2,3,4, Yarong Wang 1,3, John S. Condeelis 1,3,4, David Entenberg 1,3,4
1Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, 2Department of Radiology, Albert Einstein College of Medicine, 3Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 4Integrated Imaging Program, Albert Einstein College of Medicine

This protocol describes the use of multiphoton microscopy to perform extended time-lapse imaging of multicellular interactions in real time, in vivo at single cell resolution.

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Cancer Research

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window
Carolina Rodriguez-Tirado 1, Takanori Kitamura 5, Yu Kato 1,2, Jeffery W. Pollard 1,2,5, John S. Condeelis 3,4, David Entenberg 3,4
1Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 2Department of Obstetrics/Gynecology and Woman’s Health, Albert Einstein College of Medicine, 3Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, 4Gruss-Lipper Biophotonics Center Integrated Imaging Program, Albert Einstein College of Medicine, 5Medical Research Council Centre for Reproductive Health, Queen’s Medical Research Institute, University of Edinburgh

This protocol describes the use of multiphoton microscopy to perform long-term high-resolution, single cell imaging of the intact lung in real time using a vacuum stabilized imaging window.

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Cancer Research

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
George S. Karagiannis 1,2,3, Jessica M. Pastoriza 1,2,4, Lucia Borriello 1,2, Rojin Jafari 1,2, Anouchka Coste 1,2,4, John S. Condeelis 1,2,3,4, Maja H. Oktay 1,2,3,5, David Entenberg 1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 3Integrated Imaging Program, Albert Einstein College of Medicine, 4Department of Surgery, Montefiore Medical Center, 5Department of Pathology, Montefiore Medical Center

We describe two methods for assessing transient vascular permeability associated with tumor microenvironment of metastasis (TMEM) doorway function and cancer cell intravasation using intravenous injection of high-molecular weight (155 kDa) dextran in mice. The methods include intravital imaging in live animals and fixed tissue analysis using immunofluorescence.

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Cancer Research

A Permanent Window for Investigating Cancer Metastasis to the Lung
Lucia Borriello *1,2, Brian Traub *1,2,3, Anouchka Coste 1,2,3, Maja H. Oktay 1,2,4,5, David Entenberg 1,2,4
1Department of Anatomy and Structural Biology, Einstein College of Medicine / Montefiore Medical Center, 2Gruss-Lipper Biophotonics Center, Einstein College of Medicine / Montefiore Medical Center, 3Department of Surgery, Einstein College of Medicine / Montefiore Medical Center, 4Integrated Imaging Program, Einstein College of Medicine / Montefiore Medical Cente, 5Department of Pathology, Einstein College of Medicine / Montefiore Medical Center

Here, we present a protocol for the surgical implantation of a permanently indwelling optical window for the murine thorax, which enables high-resolution, intravital imaging of the lung. The permanence of the window makes it well-suited to the study of dynamic cellular processes in the lung, especially those that are slowly evolving, such as metastatic progression of disseminated tumor cells.

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Cancer Research

Stabilized Window for Intravital Imaging of the Murine Pancreas
Jakeb Petersen *1,2,3, Wei Du *4,5, Christian Adkisson 1,4, Claudia Gravekamp 6,7, Maja H. Oktay 2,3,4,7,8,9, John Condeelis 1,3,4,7,8,9,10, Nicole C. Panarelli 2,3,7, John C. McAuliffe 1,2,3,7,8, David Entenberg 2,3,4,7,8,9
1Department of Surgery, Einstein College of Medicine / Montefiore Medical Center, 2Department of Pathology, Einstein College of Medicine / Montefiore Medical Center, 3Integrated Imaging Program for Cancer Research, Einstein College of Medicine / Montefiore Medical Center, 4Department of Anatomy and Structural Biology, Einstein College of Medicine / Montefiore Medical Center, 5Breast Center, Peking University People's Hospital, 6Department of Microbiology & Immunology, Einstein College of Medicine / Montefiore Medical Center, 7Montefiore Einstein Cancer Center, Einstein College of Medicine / Montefiore Medical Center, 8Cancer Dormancy and Tumor Microenvironment Institute, Albert Einstein College of Medicine / Montefiore Medical Center, 9Gruss-Lipper Biophotonics Center, Einstein College of Medicine / Montefiore Medical Center, 10Department of Cell Biology, Einstein College of Medicine / Montefiore Medical Center

We present a protocol for the surgical implantation of a stabilized indwelling optical window for subcellular-resolution imaging of the murine pancreas, allowing serial and longitudinal studies of the healthy and diseased pancreas.

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