Fondazione Edmund Mach
2 ARTICLES PUBLISHED IN JoVE
Biology
High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
Mingai Li 1, Jiamei Yu 1, Enrico Barbaro 1, Claudio Varotto 1
1Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach
A high-throughput protocol for the surface sterilization of Arabidopsisthaliana (Arabidopsis) seeds is provided, optimizing the liquid handling steps with a simple suction device constructed with a vacuum pump. Hundreds of seed samples can be surface-sterilized in one day.
Biology
Methods for Rearing the Parasitoid Ganaspis brasiliensis, a Promising Biological Control Agent for the Invasive Drosophila suzukii
Marco Valerio Rossi-Stacconi 1, Xingeng Wang 2, Amanda Stout 2, Lorenzo Fellin 1,3, Kent M. Daane 4, Antonio Biondi 5, Judith M. Stahl 4, Matthew L. Buffington 6, Gianfranco Anfora 1,3, Kim A. Hoelmer 2
1Research and Innovation Centre, Fondazione Edmund Mach, 2Beneficial Insects Introduction Research Unit, Agricultural Research Service, United States Department of Agriculture, 3Center for Agriculture, Food and Environment, University of Trento, 4Department of Environmental Science, Policy and Management, University of California Berkeley, 5Department of Agriculture, Food and Environment, University of Catania, 6Systematic Entomology Laboratory, Agricultural Research Service, United States Department of Agriculture
Ganaspis brasiliensis-a larval parasitoid of Drosophila suzukii (a global invasive fruit crop pest)-has been approved or is considered for introduction into Europe and the United States for biological control of this pest. This article provides protocols for both small-scale and large-scale rearing of this parasitoid.
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