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Fondazione Edmund Mach

2 ARTICLES PUBLISHED IN JoVE

Biology

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Mingai Li¹, Jiamei Yu¹, Enrico Barbaro¹, Claudio Varotto¹

¹Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach

A high-throughput protocol for the surface sterilization of Arabidopsisthaliana (Arabidopsis) seeds is provided, optimizing the liquid handling steps with a simple suction device constructed with a vacuum pump. Hundreds of seed samples can be surface-sterilized in one day.

Biology

Methods for Rearing the Parasitoid Ganaspis brasiliensis, a Promising Biological Control Agent for the Invasive Drosophila suzukii

Marco Valerio Rossi-Stacconi¹, Xingeng Wang², Amanda Stout², Lorenzo Fellin^1,3, Kent M. Daane⁴, Antonio Biondi⁵, Judith M. Stahl⁴, Matthew L. Buffington⁶, Gianfranco Anfora^1,3, Kim A. Hoelmer²

¹Research and Innovation Centre, Fondazione Edmund Mach, ²Beneficial Insects Introduction Research Unit, Agricultural Research Service, United States Department of Agriculture, ³Center for Agriculture, Food and Environment, University of Trento, ⁴Department of Environmental Science, Policy and Management, University of California Berkeley, ⁵Department of Agriculture, Food and Environment, University of Catania, ⁶Systematic Entomology Laboratory, Agricultural Research Service, United States Department of Agriculture

Ganaspis brasiliensis-a larval parasitoid of Drosophila suzukii (a global invasive fruit crop pest)-has been approved or is considered for introduction into Europe and the United States for biological control of this pest. This article provides protocols for both small-scale and large-scale rearing of this parasitoid.